Website-based online analysis for both Raw RNAseq data and downstream analysis.

1. Raw RNAseq data analysis

We have developed a website-based online analysis tool EcoOmicsAnalyst and a docker version EcoOmicsAnalystz_local which can run in your local to conduct Raw RNAseq data analysis using Seq2Fun. It is a very easy-to-use tool and all you need to do is to upload your data to our online tool. Please check it for more details.

2. Downstream analysis

We have developed another website-based analysis tool for a comprehensive downstream data analysis and visual exploration (such as identification of differentially expressed genes, KEGG pathway analysis, GO analysis, et al).
You can submit the Seq2Fun output file S2fid_abundance_table_all_samples_submit_2_expressanalyst.txt to our website-based tool ExpressAnalyst for downstream analysis.

  1. Select Specify organism -> Seq2Fun Databases -> group
  2. Select Data type -> Bulk RNA-seq data (counts)
  3. Select ID type -> Seq2Fun Ortholog ID
  4. Select Gene-level summarization -> Sum or Mean
  5. Data File -> Choose file
  6. Click Submit button to upload;
  7. Click Proceed button to proceed to data analysis

That's it! Let us know if you have any comments.

Please be noted that this downstream analysis by our ExpressAnalyst is optional, you can always use your method to do the downstream analysis.